• Species Reactivity

  Human

• Specificity

  Detects human CDX2 in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgG    ₁ Clone # 963809

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human CDX2    
  Asn54-Thr185    
  Accession # Q99626

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  5-25 µg/mL

  See below

• 
  

• Immunocytochemistry

  5-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDX2

Human CDX2 (Caudal type homeobox transcription factor 2) is a 37 kDa member of the caudal family of homeobox proteins. It is 313 amino acids (aa) in length and contains a 167 aa caudal-like activation domain (aa 13‑180), a DNA-binding homeodomain (aa 186‑245) and two short, poly-glutamine and -proline repeats. CDX2 can exist as a dimer and is regulated by phosphorylation of a 4(x)serine motif that promotes CDX2 degradation. CDX2 promotes intestinal patterning and development. While it is necessary but not sufficient for intestinal phenotype differentiation, it will block cell proliferation on its own. Over the amino acid range used for immunization, human CDX2 is 95% and 97% aa identical to mouse and canine CDX2, respectively.

• Long Name:

  Caudal-type Homeobox Transcription Factor 2

• Entrez Gene IDs:

  1045 (Human); 12591 (Mouse); 66019 (Rat)

• Alternate Names:

  caudal type homeo box transcription factor 2; caudal type homeobox 2; Caudal-type homeobox protein 2; CDX2; CDX3; CDX-3; CDX3caudal type homeobox transcription factor 2; homeobox protein CDX-2