• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human Creatine Kinase BB in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 969409

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Creatine Kinase BB    
  Pro2-Lys381    
  Accession # P12277

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Creatine Kinase BB

Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase B (CKB), can form homodimer (BB type), and heterodimer (MB type) with creatine kinase M (CKM). MB type is found in myocardium, and homodimer BB type is found in many tissues, especially brain. The recombinant human CKB is in the BB type and its activity is measured using a phosphatase-coupled method (4).

• References:

1. Trask R.V. et al. (1988) J. Biol. Chem. 263:17142.

2. Wallimann, T. et al. (1992) Biochem, J. 281:21-40.

3. Wallimann, T. (1994) Curr. Biol. 4:42.

4. Wu, Z.L. (2011) PLoS One 6:e23172.

• Entrez Gene IDs:

  1152 (Human)

• Alternate Names:

  B-CK; CKB; Creatine Kinase BB