Mouse CCR1 Fluorescein MAb (Clone 643854) 100 TESTS

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Mouse CCR1 Fluorescein MAb (Clone 643854) 100 TESTS信息二维码

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  • 更新2024-11-27 03:31
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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Mouse

    • Specificity

      Detects mouse CCR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CCR4, 5, or 8 is observed.

    • Source

      Monoclonal Rat IgG    2B Clone # 643854

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E. coli-derived recombinant mouse CCR1    
      Extracellular regions (aa 1-34, aa 92-107, aa 172-192, aa 265-281)    
      Accession # P51675

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Fluorescein

    Applications

    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      10 µL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of CCR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse CCR1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5986F, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: CCR1

    CCR1 (C-C chemokine receptor 1; also MIP-1a receptor and CD191) is a 43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR1 is expressed on osteoclasts, IL-13  + T cells, neutrophils, bone marrow-derived mast cells, eosinophils, monocytes and vascular intimal smooth muscle cells. Multiple chemokines are reported to bind to CCR1. MIP-1a/CCL3 and RANTES/CCL5 are natural ligands, while CCL15, CCL9 and CCL23 are potent angonists after N-terminal processing. Mouse CCR1 is a 7-transmembrane protein that is 355 amino acids (aa) in length. It contains a 34 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. Over aa sequences 1-34, 92-107, 172-192 and 265-281 collectively, mouse CCR1 shares 76% and 89% aa identity with human and rat CCR1, respectively. 

    • Entrez Gene IDs:

      1230 (Human); 12768 (Mouse); 57301 (Rat)

    • Alternate Names:

      C-C chemokine receptor type 1; CC-CKR-1; CCR1; CCR-1; CD191 antigen; CD191; chemokine (C-C motif) receptor 1; CKR1; CKR-1; CMKBR1SCYAR1; CMKR1; HM145C-C CKR-1; LD78 receptor; Macrophage inflammatory protein 1-alpha receptor; MIP-1alpha-R; MIP1aR; RANTES receptor; RANTES-R










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