• Species Reactivity

  Mouse

• Specificity

  This antibody stains mouse C5L2 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Rat IgG2b Clone # 468705

• Immunogen

  Chinese hamster ovary cell line transfected with mouse C5L2    
  Met1-Val344    
  Accession # Q8BW93

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: C5L2/GPR77

C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.

• Long Name:

  C5a Anaphylatoxin Chemotactic Receptor C5L2

• Entrez Gene IDs:

  27202 (Human); 319430 (Mouse); 445269 (Rat)

• Alternate Names:

  C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77