• Species Reactivity

  Human

• Specificity

  Detects human TFEB in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 954604

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human TFEB synthetic peptide    
  Pro384-Ala446    
  Accession # P19484

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunocytochemistry

  3-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFEB

Transcription Factor EB/TFEB binds E-box (5'-CANNTG-3') where it plays a role in T cell activation through expression of CD40L and CLEAR-box (5'-GTCACGTGAC-3') sequences where it promotes expression of lysosomal genes essential for regulation of autophagy.  TFEB is localized to the cytoplasm and translocates to the nucleus under aberrant lysosomal storage conditions. Phosphorylation of S211 in an MTORC1 dependent manner sequesters TFEB in the cytoplasm through association with 14-3-3 family proteins. Inhibition of MTORC1 causes dissociation from this complex and rapid accumulation in the nucleus.

• Long Name:

  Transcription Factor EB

• Entrez Gene IDs:

  7942 (Human); 21425 (Mouse); 316214 (Rat)

• Alternate Names:

  ALPHATFEB; BHLHE35; Class E basic helix-loop-helix protein 35; T-cell transcription factor EB; TCFEB; TCFEBbHLHe35; TFEB; transcription factor EB