详细说明
- Assay TypeSolid Phase Sandwich ELISA
- Format96-well strip plate
- Sample Type & Volume Required100 µL
- Range15.60 - 1,000 pg/mL
- Sufficient MaterialsFor five 96-well plates*
- SpecificityPlease see the
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Ancillary Reagent Kit Available
DY008, DuoSet ELISA Ancillary Reagent Kit 2 - This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2HPO 4, 1.5 mM KH 2PO 4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # ), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H 2O 2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )
Stop Solution: 2 N H 2SO 4 (Catalog # )
Microplates: R&D Systems (Catalog # ), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
- StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: sFRP-5
Secreted Frizzled related proteins are secreted glycoproteins with homology to the Wnt-binding domain of the Frizzled family of transmembrane receptors. sFRPs contain an N-terminal Frizzled-like cysteine-rich domain (CRD) with 10 conserved cysteines and a C-terminal heparin-binding region. sFRPs function as soluble antagonists of Wnt signaling by binding directly to Wnt proteins and preventing their interactions with Frizzled receptors.
- Entrez Gene IDs:6425 (Human);
- Long Name:Secreted Frizzled Related Protein 5
- Aliases:frizzled-related protein 1b; FRP1B; FRP-1b; SARP3; SARP-3; secreted apoptosis related protein 3; secreted apoptosis-related protein 3; secreted frizzled-related protein 5; sFRP5; sFRP-5
400-6226-992