• Species Reactivity

  Rabbit

• Specificity

  Detects rabbit IL-4 in ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 855824

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant rabbit IL-4    
  Arg25-Ser147    
  Accession # Q9MZR8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Neutralization

  Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The Neutralization Dose (ND    ₅₀) is typically 0.25-1.25 μg/mL in the presence of 100 ng/mL Recombinant Rabbit IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa ‑ 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Rabbit IL-4 is synthesized with a 24 aa signal sequence (5). Mature rabbit IL-4 shares 47%, 56%, 39% and 40% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6‑8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4⁺ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 ‑ 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

• References:

1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.

2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.

3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.

4. Redfield, C. et al. (1991) Biochemistry 30:11029.

5. Perkins, H.D. et al. (2000) Cytokine 12:555.

6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.

7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.

8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.

9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.

10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.

11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.

12. Corthay, A. (2006) Scand. J. Immunol. 64:93.

13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.

14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.

15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

• Long Name:

  Interleukin 4

• Entrez Gene IDs:

  3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

• Alternate Names:

  B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra