• Species Reactivity

  Mouse

• Specificity

  Detects mouse PRDM14 in ELISA.

• Source

  Monoclonal Rat IgG    _2B Clone # 856235

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse PRDM14    
  Met1-Glu191    
  Accession # E9Q3T6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRDM14

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 243 – 360) and six zinc finger repeats (aa 390 - 558). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Within aa 1-191, mouse PRDM14 shares 46% aa sequence identity with human PRDM14.

• Long Name:

  PR Domain Containing 14

• Entrez Gene IDs:

  63978 (Human); 383491 (Mouse)

• Alternate Names:

  MGC59730; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRDM14; PR-domain zinc finger protein 14