详细说明
- Species ReactivityHuman, Mouse
- SpecificityDetects human Nephronectin in ELISAs and Western blots.
- SourceMonoclonal Mouse IgG 2A Clone # 859326
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenChinese hamster ovary cell line CHO-derived recombinant human Nephronectin
Glu20-Arg565
Accession # Q6UXI9 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot2 µg/mLSee below
- Immunohistochemistry5-25 µg/mLSee below
Western Blot | Detection of Human Nephronectin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse Nephronectin Monoclonal Antibody (Catalog # MAB8025) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for Nephronectin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunohistochemistry | Nephronectin in Mouse Embryonic Intestine. Nephronectin was detected in immersion fixed frozen sections of mouse embryonic intestine (13 d.p.c.) using Mouse Anti-Human/Mouse Nephronectin Monoclonal Antibody (Catalog # MAB8025) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # ). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to connective tissue. View our protocol for . |
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Entrez Gene IDs:255743 (Human); 114249 (Mouse)
- Alternate Names:EGFL6L; Nctn; Nephronectin; NPNT; POEM