• Species Reactivity

  Rat

• Specificity

  Detects rat beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in ELISA.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 882302

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat beta ‑1,3‑Glucuronyltransferase 1/B3GAT1    
  Asp75-Ile347    
  Accession # NP_445455

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 1-4GlcNAc-) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK-1 epitope, a unique trisaccharide structure, HSO₃-3GlcA beta 1-3Gal beta 1-4GlcNAc- (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3).The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell‑cell and cell‑substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single‑pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

• References:

1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.

2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.

3. Kakuda, S. et al. (2005) Glycobiology 2:203.

4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.

5. Wu, Z.L. et al. (2011) Glycobiology 21:727.

• Long Name:

  UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase

• Entrez Gene IDs:

  27087 (Human); 76898 (Mouse); 117108 (Rat)

• Alternate Names:

  B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Beta-1,3-glucuronyltransferase 1; CD57; EC 2.4.1.135; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCATP; GlcAT-P; GLCATPGLCUATP; GLCUATP; glcUAT-P; Glucuronosyltransferase P; HNK1; LEU7; NK1; NK-1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase