• Species Reactivity

  Rat

• Specificity

  Detects rat CCR9 in direct ELISAs. Stains rat CCR9 transfectants but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Mouse IgG    _2B Clone # 882416

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with rat CCR9

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR9

CCR9 is an approximately 42 kDa (predicted) seven transmembrane domain receptor for the chemokine CCL25/TECK. It is expressed on CD8  ⁺ T cells, monocytes, dendritic cells, and IgA  ⁺ plasma cells. It plays a role in oral and gut tolerance, the development of g/d T cells, and the thymic recruitment of hematopoietic progenitor cells. CCR9  ⁺ cells limit inflammatory cell infiltration in rheumatoid arthritis, hepatitis, colitis, and inflammatory bowel disease, but exacerbate it in atherosclerosis. In the brain, CCR9 is expressed on CA1 region pyramidal neurons in the hippocampus and is upregulated on microglia during   T. gondii infection. CCR9 is also upregulated in a variety of cancers where it promotes tumor cell metastasis. Rat CCR9 shares 86% and 98% amino acid sequence identity with human and mouse CCR9, respectively.

• Entrez Gene IDs:

  10803 (Human); 12769 (Mouse); 282832 (Rat)

• Alternate Names:

  C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR9; CCR-9; CD199; CDw199 antigen; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6G-protein coupled receptor 28