详细说明
- Species ReactivityHuman
- SpecificityDetects human CEBP epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEBP alpha, beta, delta, gamma, or zeta is observed.
- SourceMonoclonal Mouse IgG 1 Clone # 695345
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human CEBP epsilon
Thr151-Arg210
Accession # Q15744 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot0.5 µg/mLSee below
- Immunocytochemistry8-25 µg/mLSee below
Western Blot | Detection of Human CEBP epsilon by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human CEBP epsilon Monoclonal Antibody (Catalog # MAB6726) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for CEBP epsilon at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunocytochemistry | CEBP epsilon in HeLa Human Cell Line. CEBP epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human CEBP epsilon Monoclonal Antibody (Catalog # MAB6726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # ) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for . |
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:CCAAT/enhancer-Binding Protein epsilon
- Entrez Gene IDs:1053 (Human); 110794 (Mouse); 25410 (Rat)
- Alternate Names:C/EBP-epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon; CEBP epsilon; CEBPE; CRP1C/EBP epsilon