详细说明
- Species ReactivityHuman, Mouse, Rat
- SpecificityDetects human PP2C epsilon /PPPM1L in direct ELISAS and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PP2C is observed.
- SourceMonoclonal Mouse IgG 2B Clone # 700612
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human PP2C epsilon /PPPM1L
Asp43-Arg178
Accession # Q5SGD2 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot0.5 µg/mLSee below
Western Blot | Detection of Human, Mouse, and Rat PP2C epsilon /PPM1L by Western Blot. Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Nb2‑11 rat lymphoma cell line, Neuro‑2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human PP2C epsilon /PPM1L Monoclonal Antibody (Catalog # MAB6914) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for PP2C epsilon /PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:Protein Phosphatase 2C alpha
- Entrez Gene IDs:151742 (Human); 242083 (Mouse); 310506 (Rat)
- Alternate Names:EC 3.1.3; EC 3.1.3.16; MGC132545; MGC132547; PP2C epsilon; PP2CE; PP2CEProtein phosphatase 2C isoform epsilon; PP2C-epsilon; PPM1L; PPM1-LIKE; protein phosphatase 1 (formerly 2C)-like; protein phosphatase 1L; Protein phosphatase 1-like; Protein phosphatase 2C epsilon isoform; protein phosphatase, Mg2+/Mn2+ dependent, 1L