• Species Reactivity

  Human

• Specificity

  Detects human STIM1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human STIM2 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 705129

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human STIM1    
  Leu23-Thr182    
  Accession # Q13586

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STIM1

STIM-1 (stromal interaction molecule 1), previously called GOK, is a 90 kDa type I transmembrane protein of the endoplasmic reticulum (ER). When STIM-1 senses depletion of calcium in the ER via its EF-hand domain (aa 63-98), it interacts with the plasma membrane Ca2  ⁺ release-activated Ca2  ⁺ (CRAC) channel Orai1, increasing Ca2  ⁺ influx. The human STIM-1 extracellular/lumenal domain (aa 23-213) contains an EF hand Ca2  ⁺-binding motif and a SAM (sterile a-motif) multimerization domain. A potential isoform is truncated at aa 491. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2). Within the region used as an immunogen, human STIM1 shares 95% amino acid identity with mouse and rat STIM1.

• Long Name:

  Stromal Interaction Molecule 1

• Entrez Gene IDs:

  6786 (Human); 20866 (Mouse); 361618 (Rat)

• Alternate Names:

  GOK; GOKD11S4896E; STIM1; stromal interaction molecule 1