详细说明
- Species ReactivityHuman
- SpecificityDetects human Troponin I in direct ELISAs.
- SourceRecombinant Monoclonal Rabbit IgG Clone # 1188B
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenPeptide 3
Accession # P19429 - FormulationSupplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot1:1000 dilutionSee below
- Simple Western1:100 dilutionSee below
- Immunocytochemistry1:100 dilutionSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Western Blot | Detection of Human, Mouse, and Rat Troponin I by Western Blot. Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for Troponin I at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
| Immunocytochemistry | Troponin I in Human Cardiomyocytes. Troponin I was detected in immersion fixed human embryonic stem cells, differentiated into cardiomyocytes using the StemXVivo Cardiomyocyte Differentiation Kit (Catalog # ), using Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594) at 1:100 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Rabbit IgG Secondary Antibody (green; Catalog # ). Cardiac Troponin T was also detected in cardiomyocytes using Mouse Anti-Human Cardiac Troponin T Monoclonal Antibody (Catalog # ). Cells were co-stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining of Troponin I was localized to cardiac muscle fibrils. View our protocol for . |
| Simple Western | Detection of Human Troponin I by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.1 mg/mL. A specific band was detected for Troponin I at approximately 25-35 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Troponin I Monoclonal Antibody (Catalog # MAB8594). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Troponin I
Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2 (encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.
- Entrez Gene IDs:7137 (Human); 21954 (Mouse); 29248 (Rat)
- Alternate Names:CMH7; TNNC1; TNNI3; Troponin I
4006226992