• Species Reactivity

  Mouse

• Specificity

  Detects mouse S1P    ₅/EDG-8 in Western blots.    
   

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1196A

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Mouse S1P    ₅/EDG-8 peptide corresponding to the N-terminal extracellular sequence    
  Accession # Q91X56

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P5/EDG-8

S1P  ₅ (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P  ₁ -S1P  ₄ (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P  ₅ is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P  ₅ is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C  ⁺ (in human CD14  ⁺CD16  ^-) monocytes, pre- and mature oligodendrocytes, and CD27  ^-CD116  ⁺ (mature) NK cells. S1P  ₅ appears to play a role in cell trafficking. On monocytes and NK cells, S1P  ₅ promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P  ₅ also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P  ₅ appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P  ₅ shares 96% and 94% aa sequence identity with rat and human S1P  ₅ extracellular domains, respectively.

• Long Name:

  Endothelial Differentiation Gene 8

• Entrez Gene IDs:

  53637 (Human); 94226 (Mouse); 60399 (Rat)

• Alternate Names:

  EDG8; Edg-8; EDG8S1P receptor Edg-8; Endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; LPB4; S1P receptor 5; S1P5; S1PR5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor EDG8; Sphingosine 1-phosphate receptor Edg-8; sphingosine-1-phosphate receptor 5; SPPR-1; SPPR-2