详细说明
- SpecificityDetects N-terminal and C-terminal HA-tagged proteins in Western blot.
- SourceRecombinant Monoclonal Rabbit IgG Clone # 1049F
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenHA Tag peptide
Accession # ABB51961 - FormulationSupplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot0.1 µg/mLSee below
- Simple Western1 µg/mLSee below
- Immunoprecipitation1-4 µg/100 µg cell lysateHEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA, .
- Immunocytochemistry8-25 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Western Blot | Detection of HA-tagged proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA, C-terminal HA-tagged TRANCE, and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). Specific bands were detected for HA-tagged proteins at approximately 70 kDa, 40 kDa, and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
| Immunocytochemistry | HA Tag in NS0 Mouse Cell Line. HA was detected in immersion fixed NS0 mouse myeloma cell line transfected with HA-tagged WISP-2 using Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for . |
| Simple Western | Detection of HA-tagged proteins by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5 and N-terminal HA-tagged MICA, loaded at 0.1 mg/mL. Specific bands were detected for HA-tagged proteins at approximately 87 and 106 kDa (as indicated) using 1 µg/mL of Rabbit Anti-HA Monoclonal Antibody (Catalog # MAB0601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: HA Tag
- Entrez Gene IDs:ABB51961.1 (Viral)
- Alternate Names:HA Epitope Tag; HA Tag; HA; Hemagglutinin
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