详细说明
- SpecificityDetects N-terminal,C-terminal, and Internal Sequence HA-tagged proteins in Western blot.
- SourceRecombinant Monoclonal Mouse IgG 1 Clone # 912426
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenHA Tag peptide
Accession # ABB51961 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot0.5 µg/mLSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- Immunocytochemistry8-25 µg/mLSee below
- Intracellular Staining by Flow Cytometry0.25 µg/10 6 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Western Blot | Detection of HA-taggedproteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged LGI-2, Internal Sequence HA-tagged CIGALT, and C-terminal HA-tagged FBLN. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-HA Peptide Monoclonal Antibody (Catalog # MAB6875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). Specific bands were detected for HA-tagged proteins at approximately 60, 37, and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
| Immunocytochemistry | HA Tag in HEK293 Human Cell Line. HA Tag was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-HA Tag Monoclonal Antibody (Catalog # MAB6875) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). View our protocol for . |
| Intracellular Staining by Flow Cytometry | Detection of HA in HEK293 Human Cell Line Transfected with HA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with HA-tagged proteins was stained with Mouse Anti-HA Monoclonal Antibody (Catalog # MAB6875, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # ). |
Preparation and Storage
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HA Tag
- Entrez Gene IDs:ABB51961.1 (Viral)
- Alternate Names:HA Epitope Tag; HA Tag; HA; Hemagglutinin
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