详细说明
- Species ReactivityRat
- SpecificityDetects rat EphB1 in direct ELISAs.
- SourceMonoclonal Mouse IgG 2A Clone # 917118
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant rat EphB1
Met1-Ser538
Accession # P09759 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Immunohistochemistry8-25 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Immunohistochemistry | EphB1 in Rat Brain. EphB1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Mouse Anti-Rat EphB1 Monoclonal Antibody (Catalog # MAB5422) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in small-sized neurons. View our protocol for . |
Preparation and Storage
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphB1
EphB1, also known as Elk, Cek6, Net, and Hek6, is a type I transmembrane receptor tyrosine kinase with two extracellular fibronectin type III domains. EphB1 binds Ephrin B1, B2, and B3 and plays an important role in nervous system development and axonal migration. Within the extracellular domain, rat EphB1 shares 99% amino acid sequence identity with human and mouse EphB1.
- Long Name:Eph Receptor B1
- Entrez Gene IDs:2047 (Human); 270190 (Mouse); 24338 (Rat)
- Alternate Names:Cek6; EC 2.7.10; EC 2.7.10.1; EK6; Elk; EPH receptor B1; eph tyrosine kinase 2; EphB1; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2FLJ37986; Hek6; Net; NETHek6; soluble EPHB1 variant 1; Tyrosine-protein kinase receptor EPH-2
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