详细说明
- Species ReactivityHuman
- SpecificityDetects human Fc epsilon RI alpha in direct ELISAs.
- SourceMonoclonal Mouse IgG 2B Clone # 773704
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human Fc epsilon RI alpha
Val26-Gln205
Accession # P12319 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Flow Cytometry2.5 µg/10 6 cellsSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
- NeutralizationIn a functional ELISA, 0.08-4 μg/mL of this antibody will block 50% of the binding of 300 ng/mL of human IgE to immobilized Recombinant Human Fc epsilon RI alpha (Catalog # ) coated at 2 μg/mL (100 μL/well). At 40 μg/mL, this antibody will block >90% of the binding.
| Flow Cytometry | Detection of Fc epsilon RI alpha in Human blood cells by Flow Cytometry. Human peripheral blood cells were stained with Mouse Anti-Human ENPP‑3/CD203c APC-conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human Fc epsilon RI alpha Monoclonal Antibody (Catalog # MAB6678) or (B) Mouse IgG2B Isotype Control (Catalog # ) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). |
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The alpha subunit of the high affinity IgE receptor (Fc epsilon RI alpha or Fc epsilon RIA) is an IgE‑binding type I transmembrane glycoprotein of the multichain immune recognition (MIRR) family (1, 2). The receptor, Fc epsilon RI, is a tetrameric complex of one alpha, one beta and two gamma subunits ( alpha beta gamma 2) on mast cells and basophils (1). An alternate trimeric form ( alpha gamma 2) is expressed on human, but not rodent, mast cells, basophils, eosinophils and professional antigen presenting cells (3). While the gamma subunit is essential for expression of Fc epsilon RI alpha on the cell surface and for cell signaling, the beta subunit, when present, increases the halflife of the Fc epsilon RI complex on the cell surface (3, 4). An isoform of the beta subunit, beta T, blocks processing of the alpha subunit and its cell surface expression (2, 3, 5). Human Fc epsilon RI alpha cDNA encodes 257 amino acids (aa) including a 25 aa signal sequence, a 180 aa extracellular domain containing two Ig‑like domains that bind IgE and an endoplasmic reticulum retention motif, a 21 aa transmembrane domain with a charged amino acid (Asp219) that contributes to intracellular transport, and a 32 aa cytoplasmic sequence (1, 3, 6). Human Fc epsilon RI alpha shares 50‑62% aa sequence identity with mouse, rat, equine, ovine, bovine, porcine and canine Fc epsilon RI alpha. Binding of IgE alone increases surface expression of Fc epsilon RI, while crosslinking of IgE/Fc epsilon RI complexes by IgE ligands (allergens) initiates receptor internalization and signaling (2, 4, 5). Mast cell and basophil activation by IgE/Fc epsilon RI crosslinking causes degranulation, releasing histamine, leukotrienes, prostaglandins, and other mediators of immediate‑type and late‑phase allergic reactions. Circulating autoantibodies that crosslink Fc epsilon RI alpha are often found in patients with chronic urticaria (7). Fc epsilon RI on human antigen presenting cells mediates uptake and processing of allergens for presentation by class II MHC (2, 3). Fc epsilon RI expression on human DC and Langerhans cells is up‑regulated during allergic reactions (atopy) and correlates with serum IgE concentration (3).
- References:
- Shimizu, A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1907.
- Abramson, J. and I. Pecht (2007) Immunol. Rev. 217:231.
- Kraft, S. and J-P. Kinet (2007) Nat. Rev. Immunol. 7:365.
- Yamasaki, S. and T. Saito (2008) J. Pharmacol. Sci. 106:336.
- Brenzovich, J. et al. (2009) J. Leukoc. Biol. 86:1351.
- Cauvi, D.M. et al. (2006) J. Biol. Chem. 281:10448.
- Kikuchi, Y. et al. (2001) J. Allergy Clin. Immunol. 107:1056.
- Long Name:Fc epsilon Receptor I alpha
- Entrez Gene IDs:2205 (Human); 14125 (Mouse); 25047 (Rat)
- Alternate Names:Fc epsilon RI alpha; FCE1A; FCER1A; FcERI; FceRIa
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