详细说明
- Species ReactivityHuman
- SpecificityDetects human Eps15 in direct ELISAs and Western blots.
- SourceRecombinant Monoclonal Rabbit IgG Clone # 1261C
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenE.coli-derived recombinant human Eps15
Ala448-Thr579
Accession # P42566 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot0.1 µg/mLSee below
- Simple Western1 µg/mLSee below
- Immunocytochemistry1-25 µg/mLSee below
- Intracellular Staining by Flow Cytometry0.25 µg/10 6 cellsSee below
Western Blot | Detection of Human Eps15 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Eps15 Monoclonal Antibody (Catalog # MAB8480) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for Eps15 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunocytochemistry | Eps15 in HeLa Human Cell Line. Eps15 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Eps15 Monoclonal Antibody (Catalog # MAB8480) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for . |
Intracellular Staining by Flow Cytometry | Detection of Eps15 in U‑118‑MG Human Cell Line by Flow Cytometry. U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Rabbit Anti-Human Eps15 Monoclonal Antibody (Catalog # MAB8480, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # ). View our protocol for . |
Simple Western | Detection of Human Eps15 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, A431 human epithelial carcinoma cell line, and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Eps15 at approximately 150 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Eps15 Monoclonal Antibody (Catalog # MAB8480). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- Long Name:EGFR Pathway Substrate 15
- Entrez Gene IDs:2060 (Human); 13858 (Mouse); 313474 (Rat)
- Alternate Names:AF1P; AF-1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor pathway substrate 15; epidermal growth factor receptor substrate 15; Eps15; MLLT5; Protein AF-1p; Protein Eps15