• Species Reactivity

  Human

• Specificity

  Detects human Eps15 in direct ELISAs and Western blots.    
   

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1261C

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  E.coli-derived recombinant human Eps15    
  Ala448-Thr579    
  Accession # P42566

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Simple Western

  1 µg/mL

  See below

• 
  

• Immunocytochemistry

  1-25 µg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Eps15

Eps15 (Epidermal growth factor receptor substrate 15) is a 138-140 kDa member of the Eps family of proteins. Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionary conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet aspartate-prolinephenylalanine that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin. Eps15 binds to AP-2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles.The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and thus implicated in different facets of endosomal trafficking. Human EPS15 has been mapped to chromosome 1p31- p32, a region displaying several non-random chromosomal abnormalities, including deletions in neuroblastoma and translocation in acute lymphoblastic and myeloid leukemias. Over aa 492-579, human EPS15 shares 76% aa identity with mouse EPS15.

• Long Name:

  EGFR Pathway Substrate 15

• Entrez Gene IDs:

  2060 (Human); 13858 (Mouse); 313474 (Rat)

• Alternate Names:

  AF1P; AF-1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor pathway substrate 15; epidermal growth factor receptor substrate 15; Eps15; MLLT5; Protein AF-1p; Protein Eps15