Mouse Cadherin-17 MAb (Clone 1262C) 100 UG

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Mouse Cadherin-17 MAb (Clone 1262C) 100 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Mouse

    • Specificity

      Detects mouse Cadherin-17 in direct ELISAs and Western blots.    
       

    • Source

      Recombinant Monoclonal Rabbit IgG Clone # 1262C

    • Purification

      Protein A or G purified from cell culture supernatant

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17    
      Glu22-Met786    
      Accession # Q9R100

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 µg/mL

      See below


    • Simple Western

      1 µg/mL

      See below


    • Immunohistochemistry

      1-25 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Mouse Cadherin‑17 by Western Blot. Western blot shows lysates of mouse colon tissue and mouse spleen tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for Cadherin‑17 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Immunohistochemistry      
         

    Cadherin‑17 in Mouse Intestine. Cadherin‑17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes. View our protocol for .

    Simple Western      
         

    Detection of Mouse Cadherin‑17 by Simple WesternTM. Simple Western lane view shows lysates of mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Cadherin‑17 at approximately 153 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Mouse Cadherin‑17 Monoclonal Antibody (Catalog # MAB8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Cadherin-17

    Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa.

    • Entrez Gene IDs:

      1015 (Human); 12557 (Mouse)

    • Alternate Names:

      cadherin 17, LI cadherin (liver-intestine); cadherin; cadherin-16; Cadherin17; Cadherin-17; CDH16; CDH17; FLJ26931; HPT-1 cadherin; HPT1; HPT-1; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024




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