详细说明
- Species ReactivityHuman
- SpecificityDetects human ERMAP in direct ELISAs.
- SourceMonoclonal Mouse IgG 1 Clone # 767505
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human ERMAP
His30-Ala155
Accession # Q96PL5 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Flow Cytometry2.5 µg/10 6 cellsSee below
- CyTOF-readyReady to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Flow Cytometry | Detection of ERMAP in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB49281, filled histogram) or isotype control antibody (Catalog # , open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). |
Preparation and Storage
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERMAP
ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60-66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors, and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30-155) that contains one V-type Ig-like domain (aa #30-140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103-475. Over aa 30-155, human ERMAP shares 56% aa sequence identity with mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.
- Long Name:Erythroblast Membrane-associated Protein
- Entrez Gene IDs:114625 (Human); 27028 (Mouse); 298485 (Rat)
- Alternate Names:ERMAP; erythroblast membrane-associated protein (RD and SC blood groups); erythroblast membrane-associated protein (Scianna blood group); erythroblast membrane-associated protein; erythroid membrane-associated protein; hERMAP; MGC118810; MGC118811; PRO2801; Radin blood group (Rd); Radin blood group antigen; Radin blood group; RD; RDMGC118812; SC; Scianna blood group (Sc); Scianna blood group antigen; Scianna blood group; SCMGC118813
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