• Species Reactivity

  Mouse

• Specificity

  Detects mouse Thrombospondin‑4 in ELISA.

• Source

  Monoclonal Rat IgG    _2A Clone # 893655

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse Thrombospondin‑4    
  Met1-Asn963    
  Accession # Q9Z1T2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Mouse Thrombospondin-4 (TSP-4) is a 120-130 kDa adhesive glycoprotein that belongs to the thrombospondin family of proteins.  It is 963 amino acids (aa) in length and highly modular in structure, containing one Lamnin G-like domain, four EGF-like domains and eight type 3 TSP domains.  It circulates as a disulfide-linked homopentamer, and is up-regulated in the heart in response to ischemic injury and pathology. Mature mouse TSP-4 is 93% aa identical to human TSP4.

• Entrez Gene IDs:

  7060 (Human); 21828 (Mouse)

• Alternate Names:

  THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4