• Species Reactivity

  Mouse

• Specificity

  Detects mouse DC‑SIGN/CD209 in direct ELISAs.

• Source

  Recombinant Monoclonal Rat IgG    _2B Clone # 902404

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with mouse DC‑SIGN/CD209    
  Met1-Lys238    
  Accession # Q91ZX1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 µg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca  ²⁺-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

• References:

1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.

2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.

3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.

4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.

5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.

6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.

• Long Name:

  Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1

• Entrez Gene IDs:

  30835 (Human); 170786 (Mouse)

• Alternate Names:

  CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1