详细说明
- Species ReactivityHuman
- SpecificityDetects human Serum Amyloid A1 in direct ELISAs.
- SourceMonoclonal Mouse IgG 3 Clone # 902738
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human Serum Amyloid A1
Accession # P0DJI8 - FormulationLyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Immunohistochemistry8-25 µg/mLSee below
| Immunohistochemistry | Serum Amyloid A1 in Human Liver. Serum Amyloid A1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Amyloid A1 Monoclonal Antibody (Catalog # MAB30191) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocytes. View our protocol for . |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human serum amyloid A protein-1 (SAA-1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12 kDa, 104 amino acid (aa), nonglycosylated polypeptide that displaces apoA1 in the HDL3 complex (SwissProt #P02735). The SAA-1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA-1 gene product differs from the SAA-2 gene product by only seven amino acids. Circulating SAA-1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA-1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA-1, respectively.
- Entrez Gene IDs:6288 (Human); 20208 (Mouse)
- Alternate Names:MGC111216; PIG4; SAA; SAA1; SAA2; serum amyloid A protein; Serum Amyloid A1; TP53I4; tumor protein p53 inducible protein 4
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