详细说明
Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 3B in direct ELISAs.
Source
Monoclonal Rat IgG 2A Clone # 904201
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 3B
Pro26-Pro722
Accession # Q62177Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Mouse Semaphorin 3B by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). A specific band was detected for Semaphorin 3B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Immunocytochemistry | Semaphorin 3B in NS0 Mouse Cell Line. Semaphorin 3B was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse Semaphorin 3B (upper panel) or irrelevant transfectant (lower panel) using Rat Anti-Mouse Semaphorin 3B Monoclonal Antibody (Catalog # MAB5440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for . |
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 3B
Sema3b (Semaphorin 3B) is a 748 aa secreted glycoprotein. Mouse Sema3b shares 88% and 96% aa identity with human and rat Sema3b, respectively. The semaphorin family has been demonstrated to play a role in axon guidance. Additionally, Sema3b has been implicated in bone mass regulation and tumor suppression.
Entrez Gene IDs:
7869 (Human); 20347 (Mouse); 363142 (Rat)
Alternate Names:
(semaphorin) 3B; LUCA-1; Sema A(V); sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema V; SemA; SEMA3B; SEMA5; SEMA5FLJ34863; SEMAA; Semaphorin 3B; semaphorin A; semaphorin V; semaphorin-3B; semaphorin-V; SEMAV
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