详细说明
- Species ReactivityHuman, Mouse, Rat
- SpecificityDetects human, mouse, and rat Nrf2 in Western blots. In Western blots, no cross-reactivity with recombinant human Nrf1 is observed.
- SourceMonoclonal Mouse IgG 2B Clone # 383727
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot1 µg/mLSee below
Western Blot | Detection of Human Nrf2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-), treated (+) or mock treated (m) with 10 µM MG132 (Catalog # ) for 4 hours and 10 pmol siRNA. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf2 Monoclonal Antibody (Catalog # MAB3925) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for Nrf2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.
- Long Name:NF-E2-related Factor 2
- Entrez Gene IDs:4780 (Human); 18024 (Mouse); 83619 (Rat)
- Alternate Names:HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2