• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat SOX2 in Western blots.

• Source

  Monoclonal Mouse IgG    _2A Clone # 245610

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SOX2    
  Gly135-Met317    
  Accession # P48431

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Simple Western

  4 µg/mL

  See below

• 
  

• CyTOF-reported

  Zunder, E.R.     et al. (2015) Cell Stem Cell     16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).

• References:

1. Graham, V. et al. (2003) Neuron 39:749.

2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.

3. Kishi, M. et al. (2000) Development 127:791.

4. Yuan, H. et al. (1995) Genes Dev. 9:2635.

5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.

6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.

• Long Name:

  Transcription Factor SOX2

• Entrez Gene IDs:

  6657 (Human)

• Alternate Names:

  ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2