• Species Reactivity

  Human, Mouse

• Specificity

  Detects human Stathmin-2/STMN2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Stathmin-1, -3, or -4 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 684433

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Stathmin-2/STMN2    
  Tyr26-Gly179    
  Accession # Q93045

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.05 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stathmin-2/STMN2

Stathmin-2 (STMN2), also known as superior cervical ganglion 10 protein (SCG10), is a 19 kDa microtubule-destabilizing protein that is preferentially expressed in neurons. It promotes neuronal proliferation and migration as well as neurite outgrowth. Stathmin-2 contains a membrane attachment region  
(aa 1‑26), a regulatory domain (aa 39-96), and an overlapping coiled-coil domain (aa 75-179). It is subject to phosphorylation of multiple serines within the regulatory domain. Within aa 26‑179, human Stathmin-2 shares 100% and 99% aa sequence identity with mouse and rat Stathmin-2, respectively.

• Entrez Gene IDs:

  11075 (Human); 20257 (Mouse); 84510 (Rat)

• Alternate Names:

  FLJ34868; FLJ50995; neuron-specific growth-associated protein; Protein SCG10; SCG10; SCG10neuronal growth-associated protein (silencer element); SCGN10; SCGN10stathmin-2; Stathmin2; Stathmin-2; stathmin-like 2; STMN2; superior cervical ganglia, neural specific 10; Superior cervical ganglion-10 protein