• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat DRAK2 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) DRAK1, rhDAPK1, rhDAPK2, or rhDAPK3 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 685228

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human DRAK2    
  Ala87-Glu285 (predicted)    
  Accession # O94768

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DRAK2

DRAK2 (death-associated protein related kinase-2), also called STK17B (Ser/Thr kinase 17B) is a 45 kDa member of the DAPK (death-associated protein kinase) family. It is expressed mainly in lymphoid organs by mature T cells and B cells. It is autophosphorylated on Ser12 after TCR stimulation and negatively regulates TCR signaling. Ultraviolet light causes phosphorylation on Ser350 and translocation to the nucleus to stimulate apoptosis. Within the region used as an immunogen, human DRAK2 shares 95% amino acid (aa) identity with mouse and rat DRAK2 while mouse and rat DRAK2 share 99.5% aa identity.

• Long Name:

  DAP Kinase-related Apoptosis-inducing Protein Kinase 2

• Entrez Gene IDs:

  9262 (Human)

• Alternate Names:

  DAP kinase-related apoptosis-inducing protein kinase 2; DRAK2; DRAK2death-associated protein kinase-related 2; EC 2.7.11; EC 2.7.11.1; serine/threonine kinase 17b (apoptosis-inducing); serine/threonine kinase 17b; serine/threonine-protein kinase 17B; STK17B