• Specificity

  Detects Vitamin D3 in flow cytometry.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 685503

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  25-Hydroxyvitamin D    ₃  

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D3

25-hydroxy-vitamin D  ₃ (25(OH)D  ₃) is synthesized in the liver and is the primary circulating form of vitamin D.  Its blood concentration, which reflects 25(OH)D  ₃ produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status.  25(OH)D  ₃ is metabolized to 1 alpha,25(OH)  ₂D  ₃ in the  proximal tubular cells of the kidney by the enzyme 1 alpha -hydroxylase.   The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure.  It has been shown that the active steroid hormone 1 alpha,25(OH)  ₂D  ₃ is essential for life in higher animals.  Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D  ₃, 1 alpha,25(OH)  ₂D  ₃, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha -hydroxylase is present.

• Alternate Names:

  Calcifediol; Calciol; Calcitriol; Cholecalciferol; Vitamin D3