• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human p130Cas in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Ajuba is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 685718

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human p130Cas    
  Met66-Val412    
  Accession # P56945

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p130Cas

p130Cas (protein 130 kDa Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93 kDa, it runs anomalously at 105-130 kDa in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31 kDa fragment promotes anoikis and cell death. Human p130Cas is 870 amino acids (aa) in length. It contains one SH3 domain (aa 3-65), a Pro‑rich region (aa 74-87), at least 10 phosphotyrosine (aa 128-410) and four phosphoserine (aa 134-639) sites, an SH3 binding motif (aa 635-643) and a HLH dimerization segment that interacts with CASL. There are multiple splice variants. There is a 22 aa substitution for aa 1-4, an alternate start site at Met30, a deletion of aa 2-211, and a four aa substitution for aa 1-4 with a deletion of aa 65-212. Over aa 66-412, human p130Cas shares 91% aa identity with mouse p130Cas.    

• Long Name:

  Breast Cancer Anti-estrogen Resistance 1

• Entrez Gene IDs:

  9564 (Human); 12927 (Mouse); 25414 (Rat)

• Alternate Names:

  BCAR1; breast cancer estrogen resistance 1; Cas scaffolding protein family member 1; CAS1; CASFLJ45059; CASS1; CASS1CRKASbreast cancer estrogen resistance protein 1; CRKAS; Crk-associated substrate p130Cas; CRK-associated substrate; FLJ12176; p130Cas