• Species Reactivity

  Human

• Specificity

  Detects human DISC1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 685920

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human DISC1    
  Lys101-Arg260    
  Accession # Q9NR15

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DISC1

Disrupted in schizophrenia 1 (DISC1) is a 100 kDa cytoplasmic scaffold protein that is associated with the development of schizophrenia, bipolar disorder, and recurrent major depression. It plays a role in post-synaptic density development and neurogenesis as well as centrosome and microtubule dynamics. DISC1 interacts with a range of intracellular proteins including Kinesin 1, NDE1, PDE4, GSK-3 beta, GRB2, PACAP, RAC1, TNIK, and FEZ1. It contains four coiled-coil domains (aa 366-394, aa 452-505, aa 602-666, and aa 802-830) which mediate the assembly of DISC1 into a variety of multimers. DISC1 multimerization and its ability to interact with various binding parters are regulated by post-translational modifications and proteolysis. Alternate splicing generates additional isoforms of human DISC1 that are truncated before the first or following the third coiled coil domain. Within aa 101-260, human DISC1 shares approximately 43% aa sequence identity with mouse and rat DISC1.

• Long Name:

  Disrupted in Schizophrenia 1

• Entrez Gene IDs:

  27185 (Human); 244667 (Mouse); 307940 (Rat)

• Alternate Names:

  C1orf136; DISC1; disrupted in schizophrenia 1 protein; disrupted in schizophrenia 1; FLJ13381; FLJ21640; FLJ25311; FLJ41105; KIAA0457; SCZD9