• Species Reactivity

  Human, Mouse

• Specificity

  Detects mouse RBP4/Retinol‑Binding Protein 4 in direct ELISA and Western Blot. In Western blots, detects human RBP4/Retinol‑Binding Protein 4.

• Source

  Monoclonal Rat IgG2a Clone # 423619

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse RBP4/Retinol‑Binding Protein 4    
  Met1-Leu201    
  Accession # NP_035385

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with Retinol-binding Protein 4 (RBP4), also known as Plasma Retinol-binding Protein (1‑3). A prototypic member of the lipocalin superfamily, RBP4 has a beta ‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases. The amino acid sequence of mouse RBP4 is 99%, 86%, 83% and 75% identical to that of rat, human/chimpanzee, dog and chicken.

• References:

1. Zanotti, G. and R. Berni (2004) Vitamins and Hormones 69:271.

2. Newcomer, M.E. and D.E. Ong (2000) Biochim. Biophys. Acta 1482:57.

3. Yang, Q. et al. (2005) Nature 436:356.

• Entrez Gene IDs:

  5950 (Human); 19662 (Mouse); 25703 (Rat)

• Alternate Names:

  interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma