• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human MCHR1 in ELISAs. Detects human, mouse and rat MCHR1 in Western Blots    
   

• Source

  Monoclonal Mouse IgG    _2A Clone # 851047

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human MCHR1    
  Ser2-Pro113    
  Accession # Q99705

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Simple Western

  50 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCHR1

MCHR1 (melanin-concentrating hormone receptor-1), also called GPR24 (G-protein coupled receptor 24) or SLC1 (somatostatin receptor-like 1), is an approximately 44 kDa, seven-transmembrane receptor for MHC. It is most highly expressed in the brain but is also present in peripheral tissues. Engagement of MHCR1 has effects on food intake, energy homeostasis, anxiety and depression, such that rodents treated with small molecule antagonists weigh less and show reduced anxiety and depression in model systems than untreated rodents. Human MCHR1 shares 74% amino acid (aa) sequence identity with mouse MCHR1 between aa 1-113, and 82% with rat MCHR1 between aa 71-113.

• Long Name:

  Melanin-concentrating Hormone Receptor 1

• Entrez Gene IDs:

  2847 (Human); 207911 (Mouse); 83567 (Rat)

• Alternate Names:

  G protein-coupled receptor 24; GPR24; GPR24SLC-1; G-protein coupled receptor 24; MCH receptor 1; MCH1R; MCH-1R; MCH1RMCHR-1; MCHR1; MCH-R1; melanin-concentrating hormone receptor 1; MGC32129; SLC1; SLC1MCHR; Somatostatin receptor-like protein