• Species Reactivity

  Human

• Specificity

  Detects human Siglec‑6/CD327 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2A Clone # 767329

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Siglec‑6/CD327    
  Gln27-Val331    
  Accession # NP_942142

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-6/CD327

Siglecs (Sialic acid binding Ig-like Lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1-4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6-9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

• References:

1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.

2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.

3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.

4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.

5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.

6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.

7. GenBank Accession # NP_942143.

8. GenBank Accession # NP_942142.

9. GenBank Accession # NP_001236.

10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.

• Long Name:

  Sialic Acid Binding Ig-like Lectin 6

• Entrez Gene IDs:

  946 (Human)

• Alternate Names:

  CD327 antigen; CD327; CD33 antigen-like 1; CD33L1; CD33L1OBBP1CD327; CD33L2; CD33LCDW327; CDw327; OB-BP1; Obesity-binding protein 1; sialic acid binding Ig-like lectin 6; sialic acid-binding Ig-like lectin 6; Siglec6; Siglec-6