Human Jagged 1 DuoSet ELISA, 5 Plate 1 KT

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Human Jagged 1 DuoSet ELISA, 5 Plate 1 KT信息二维码

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产品介绍

    基本参数

    详细说明

    • Assay Type

      Solid Phase Sandwich ELISA

    • Format

      96-well strip plate

    • Sample Type & Volume Required

      100 µL

    • Range

      0.312 - 20 ng/mL

    • Sufficient Materials

      For five 96-well plates*

    • Specificity

      Please see the

    * Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

    Ancillary Reagent Kit Available  

    DY008, DuoSet ELISA Ancillary Reagent Kit 2 -

    This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant


     

    Product Features

    • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time

    • Development protocols are provided to guide further assay optimization

    • Assay can be customized to your specific needs

    • Economical alternative to complete kits

    Kit Content

    • Capture Antibody

    • Detection Antibody

    • Recombinant Standard

    • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

    Other Reagents Required


      PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na  2HPO  4, 1.5 mM KH  2PO  4, pH 7.2 - 7.4, 0.2 µm filtered  
     
      Wash Buffer: (Catalog # ), or equivalent  
     
      Reagent Diluent*  
     
      Blocking Buffer*  
     
      Substrate Solution: 1:1 mixture of Color Reagent A (H  2O  2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )  
     
      Stop Solution: 2 N H  2SO  4 (Catalog # )  
     
      Microplates: R&D Systems (Catalog # ), or equivalent  
     
      Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent  
     

    *For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product

    Preparation and Storage

    • Storage

      Store the unopened product at 2 - 8 °C. Do not use past expiration date.

    Background: Jagged 1

    Mammalian Jagged proteins are homologs of the Drosophila Notch ligand, Serrate, and together with Delta-like proteins, constitute ligands for the mammalian Notch proteins. Jagged 1 and 2 are 180 kDa and 150 kDa type I transmembrane glycoproteins, respectively, that possess extracellular DSL (delta, serrate, lag-2 consensus sequence) domains necessary for binding to Notch receptors, along with 15-16 calcium-binding EGF-like repeats which modulate the binding affinity. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis and development of the vasculature. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for functional Notch signaling.

    • Entrez Gene IDs:

      182 (Human); 29146 (Rat);

    • Aliases:

      AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

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