• Species Reactivity

  Human

• Specificity

  Detects human Siglec-9 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Siglec-2, -3, -5, -7 or -10 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 191240

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Siglec-9    
  Gln18-Gly348 (predicted)    
  Accession # Q9Y336

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Siglec-9

Siglecs(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs have been cloned and characterized. They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglec-5 to -11 (1‑4). To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec-3 both in their extracellular and intracellular regions. They are collectively referred to as CD33-related Siglecs. One remarkable feature of the CD33-related Siglecs is their differential expression pattern within the hematopoietic system (2, 3). This fact, together with the presence of two conserved immunoreceptor tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails, suggests that CD33-related Siglecs are involved in the regulation of cellular activation within the immune system.

The cDNA of human Siglec-9 encodes a 463 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-likeV-type domain, two Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail (5, 6). In peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8⁺ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). Siglec-9 is closely related to Siglec-7, and they share ~80% amino acid sequence identity. The gene encoding siglec-9 was mapped to chromosome 19q13.4.

• References:

1. Crocker, P.R. et al. (1998) Glycobiology 8:v.

2. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.

3. Crocker, P.R. and A. Varki (2001) Immunology 103:137.

4. Angata, T. et al. (2002) J. Biol. Chem. 277:24466.

5. Zhang, J.Q. et al. (2000) J. Biol. Chem. 275:22121.

6. Angata, T. et al. (2000) J. Biol. Chem. 275:22127.

• Long Name:

  Sialic Acid Binding Ig-like Lectin 9

• Entrez Gene IDs:

  27180 (Human)

• Alternate Names:

  CD329; CDw329; FOAP-9; OBBP-Like; Protein FOAP-9; sialic acid binding Ig-like lectin 9; sialic acid-binding Ig-like lectin 9; Siglec9; Siglec-9