• Species Reactivity

  Human

• Specificity

  Detects human Nestin.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 196908

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human Nestin

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nestin

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and   in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

• References:

1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.

2. Lendahl, U. et al. (1990) Cell 60:585.

3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.

4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.

5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.

6. Frederiksen, K. et al. (1988) Neuron 1:439.

7. Cattaneo, E. et al. (1990) Nature 347:762.

8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.

9. Rietze, R.L. et al. (2001) Nature 412:736.

10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.

11. Zulewski, H. et al. (2001) Diabetes 50:521.

12. Lumelsky, N. et al. (2001) Science 292:1389.

13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.

14. Shih, C.C. et al. (2001) Blood 98:2412.

• Entrez Gene IDs:

  10763 (Human); 18008 (Mouse); 25491 (Rat)

• Alternate Names:

  FLJ21841; NES; Nestin