• Species Reactivity

  Human

• Specificity

  Detects human Annexin A6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Annexin A1, A2, A3, A4, A8, A9, A10, A11, or A13 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 686921

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Annexin A6    
  Ala2-Asp673    
  Accession # P08133

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60-70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5‑7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

• References:

1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.

2. Crompton, M.R. et al. (1988) EMBO J. 7:21.

3. Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.

4. Grewal, T. et al. (2006) Bioessays 28:1211.

5. Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.

6. Takagi, H. et al. (2002) J. Cell Sci. 115:3309.

7. Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.

8. Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

• Entrez Gene IDs:

  309 (Human); 11749 (Mouse); 79125 (Rat)

• Alternate Names:

  67 kDa calelectrin; Annexin A6; annexin VI (p68); Annexin VI; Annexin-6; ANX6; ANXA6; calcium-binding protein p68; Calelectrin; calphobindin II; calphobindin-II; CBP68; chromobindin-20; CPB-II ; CPB-II; Lipocortin VI; p68; p70; Protein III