• Species Reactivity

  Rat

• Specificity

  Detects rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat Neuropilin-2 is observed.

• Source

  Monoclonal Mouse IgG1 Clone # 130604

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1    
  Met1-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted    
  Accession # Q9QWJ9

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuropilin-1

Neuropilin-1 (Npn-1, previously neuropilin; also CD304) is a 130‑140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1‑4). The mature 901 amino acid (aa) rat Npn-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with mouse and 93% aa identity with human, equine, bovine and canine Npn-1 (3, 4). The ECD contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII and a MAM (meprin) domain. In mouse and human, splice variants that lack the TM domain have been described and are either proven or presumed to be soluble antagonists (1, 5‑7). The sema domains of Class III secreted semaphorins such as Sema3A bind Npn-1 CUB domains (8). The heparin-binding forms of VEGF (VEGF  ₁₆₅, VEGF-B and VEGF-E), PlGF (PlGF2), and the C-terminus of Sema3 bind the F5/8 type C domains (8, 9). Npn-1 and Npn-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (10). However, only Npn-1 binds Sema3A, and only Npn-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF  ₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF  ₁₆₅, which has higher affinity for Npn-1 (11). Npn-1 is preferentially expressed in developing or remodeling arteries (1, 2). Npn-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (12).

• References:

1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 

2. Gu, C. et al. (2003) Dev. Cell 5:45. 

3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.

4. Soker, S. et al. (1998) Cell 92:735.

5. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 

6. Cackowski, F.C. et al. (2004) Genomics 84:82. 

7. Rossignol, M. et al. (2000) Genomics 70:211.

8. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.

9. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.

10. Yaron, A. et al. (2005) Neuron 45:513.

11. Narazaki, M. and G. Tosato (2006) Blood 107:3892.

12. Tordjman, R. et al. (2002) Nat. Immunol. 3:477.

• Entrez Gene IDs:

  8829 (Human); 18186 (Mouse); 246331 (Rat)

• Alternate Names:

  BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304