• Species Reactivity

  Mouse

• Specificity

  Detects mouse Neuropilin-1 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant rat (rr) Neuropilin-1 is observed and no cross-reactivity with recombinant human (rh) Neuropilin-1, rhNeuropilin-2, or rrNeuropilin-2 is observed.

• Source

  Monoclonal Rat IgG2a Clone # 761705

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Neuropilin-1    
  Phe21-Pro856    
  Accession # P97333

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  bEnd.3 mouse endothelioma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neuropilin-1

Neuropilin-1 (Nrp-1, previously neuropilin; also CD304) is a 130 - 140 kDa type I transmembrane (TM) glycoprotein that regulates axon guidance and angiogenesis (1 - 4). The full-length 923 amino acid (aa) mouse Nrp-1 contains a 623 aa extracellular domain (ECD) that shares 98% aa identity with rat and 93% aa identity with human, equine, bovine and canine Nrp-1 (3, 4). The ECD contains two N-terminal CUB domains (termed a1a2), two domains with homology to coagulation factors V and VIII (b1b2) and a MAM (meprin) domain (c). At least one splice variant that diverges at aa 587 and lacks the TM domain has been sequenced (5). This form is potentially a soluble antagonist, based on results from human Nrp-1 splice variants (1, 6 - 8). The sema domains of Class III secreted semaphorins such as Sema3A bind Nrp-1 a1a2 (9). Heparin, the heparin-binding forms of VEGF (VEGF  ₁₆₅, VEGF-B and VEGF-E), P  lGF (P  lGF2), and the C-terminus of Sema3 bind the b1b2 region (9, 10). Nrp-1 and Nrp-2 share 48% aa identity within the ECD and can form homo- and hetero-oligomers via interaction of their MAM domains (1). Neuropilins show partially overlapping expression in neuronal and endothelial cells during development (1, 2). Both neuropilins act as co-receptors with plexins, mainly plexin A3 and A4, to bind class III semaphorins that mediate axon repulsion (11). However, only Nrp-1 binds Sema3A, and only Nrp-2 binds Sema3F (1). Both are co-receptors with VEGF R2 (also called KDR or Flk-1) for VEGF  ₁₆₅ binding (1). Sema3A signaling can be blocked by VEGF  ₁₆₅, which has higher affinity for Npn-1 (12). Nrp-1 is preferentially expressed in developing or remodeling arteries (1, 2). Nrp-1 is also expressed on dendritic cells and mediates DC-induced T cell proliferation (13).

• References:

1. Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584. 

2. Gu, C. et al. (2003) Dev. Cell 5:45. 

3. He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.

4. Soker, S. et al. (1998) Cell 92:735.

5. Entrez accession #EDL11827

6. Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573. 

7. Cackowski, F.C. et al. (2004) Genomics 84:82. 

8. Rossignol, M. et al. (2000) Genomics 70:211.

9. Gu, C. et al. (2002) J. Biol. Chem. 277:18069.

10. Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.

11. Yaron, A. et al. (2005) Neuron 45:513.

12. Narazaki, M. and G. Tosato (2006) Blood 107:3892.

13. Tordjman, R. et al. (2002) Nat. Immunol. 3477.

• Entrez Gene IDs:

  8829 (Human); 18186 (Mouse); 246331 (Rat)

• Alternate Names:

  BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304