• Species Reactivity

  Human

• Specificity

  Detects human CD15/Lewis X in direct ELISAs.    
   

• Source

  Monoclonal Mouse IgM Clone # ICRF29-2

• Immunogen

  human granulocytes

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood granulocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD15/Lewis X

Lewis X (Le  ^x) is a glycan structure (Gal beta 1-4(Fuc alpha 1-3)GlcNAc beta -) that forms the terminus of N-linked carbohydrates such as the antigenic epitope Stage-Specific Embryonic Antigen-1 (SSEA-1/CD15). It is widely expressed, can be found on glycoproteins, glycolipids and proteoglycans and is a blood group antigen. Le  ^x is a marker for mouse embryonic stem and cancer cells, and is expressed on leukocytes, germ cells and neuronal stem cells in both mouse and human. Le  ^x is thought to enhance cell adhesion, either directly or by promoting integrin activation.

• Alternate Names:

  CD 15; CD15; CD-15; Lewis X; Lewis-X; LeX; SSEA-1; stage-specific embryonic antigen 1