• Species Reactivity

  Mouse

• Specificity

  Detects mouse MMR in direct ELISAs.

• Source

  Monoclonal Rat IgG1 Clone # 857615

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse MMR    
  Leu19-Ala1388    
  Accession # Q61830

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  J774A.1 mouse reticulum cell sarcoma macrophage cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) mouse CD206 precursor contains a signal sequence (19 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The mouse CD206 ECD shares 96% aa sequence identity with rat MR, and 83‑84% with human, equine, porcine and canine CD206. The cysteine-rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis  ^a and ‑Lewis  ^x (1, 7, 9). The FNII domain mediates Ca2  ⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2  ⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin-coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

• References:

1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.

2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.

3. Harris, N. et al. (1992) Blood 80:2363.

4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.

5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.

6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.

7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.

8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.

9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.

10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.

11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.

12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

• Long Name:

  Macrophage Mannose Receptor

• Entrez Gene IDs:

  4360 (Human); 17533 (Mouse); 291327 (Rat)

• Alternate Names:

  CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1