• Species Reactivity

  Human

• Specificity

  Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse EphA4, A5, A6, A7, A8, or recombinant rat EphB1 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 371805

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human EphA2    
  Gln25-Asn534    
  Accession # P29317

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EphA2

EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1, 2, 3, 4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90‑94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).

• References:

1. Poliakov, A. et al. (2004) Dev. Cell 7:465.

2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419. 

3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.

4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.

5. Bohme, B et al. (1993) Oncogene 8:2857.

6. Pandey, A. et al. (1995) Science 268:567.

7. Bartley, T.D. et al. (1994) Nature 368:558.

8. Pandey, A. et al. (1994) J. Biol. Chem. 269:30154.

9. Miao, H. et al. (2001) Nat. Cell Biol. 3:527.

10. Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793.

11. Dohn, M. et al. (2001) Oncogene 20:6503.

12. Zelinski, D.P. et al. (2001) Cancer Res. 61:2301.

13. Brantley, D.M. et al. (2002) Oncogene 21:7011.

14. Ogawa, K. et al. (2000) Oncogene 19:6043.

15. Sulman, E.P. et al. (1997) Genomics 40:371.

• Entrez Gene IDs:

  1969 (Human); 13836 (Mouse)

• Alternate Names:

  ARCC2; EC 2.7.10; EC 2.7.10.1; Eck; ECKepithelial cell receptor protein tyrosine kinase; EPH receptor A2; EphA2; ephrin type-A receptor 2; Epithelial cell kinase; Myk2; Sek2; soluble EPHA2 variant 1; Tyrosine-protein kinase receptor ECK