• Species Reactivity

  Human

• Specificity

  Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse VEGF R1, recombinant human (rh) VEGF R2, rhVEGF R3, or rhVEGF R4.

• Source

  Monoclonal Mouse IgG1 Clone # 49560

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human VEGF R1/Flt-1    
  Ser27-His687    
  Accession # NP_001153392

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  HUVEC human umbilical vein endothelial cells

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGF R1/Flt-1

Vascular Endothelial Growth Factor Receptor 1 (VEGF R1) is a receptor tyrosine kinase that is expressed primarily on endothelial cells and plays a role in vasculogenesis and angiogenesis. A soluble variant of VEGF R1 was also reported to bind VEGF and PlGF with high affinity and function as a potent VEGF antagonist.

• Long Name:

  Vascular Endothelial Growth Factor Receptor 1

• Entrez Gene IDs:

  2321 (Human); 14254 (Mouse)

• Alternate Names:

  EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1