• Species Reactivity

  Human

• Specificity

  Detects human FCRL6/FcRH6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) FcRH1, rhFcRH2, and rhFcRH4 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human FCRL6/FcRH6    
  Lys16-Leu312    
  Accession # Q6DN72

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL6/FcRH6

FCRL6 (Fc receptor-like protein 6; also FcRH6 and IFGP6) is a 66 kDa member of the Ig Superfamily. It is found on CD56dim CD16  ⁺ NK cells, CD56  ⁺ CD3  ⁺ NKT cells, gd T cells, and effector plus effector-memory CD8  ⁺ T cells. FCRL6 is not a receptor for immunoglobulin. It does, however, bind to isoforms of HLA-DR that are expressed on APCs. Given that FCRL6 contains a cytoplasmic ITIM, FCRL6 may function as an inhibitory receptor for MHC Class II.  Mature human FCRL6 is a 415 amino acid (aa) type I transmembrane protein. It contains a 288 aa extracellular region (aa 20-307) that shows three C2-type Ig-like domains (aa 20-293), and a 106 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 105-200 accompanied by a 13 aa substitution for aa 394-434; a second possesses a 15 aa substitution for aa 383-434; and a third shows an alternative start site seven aa upstream of the standard site accompanied by a 13 aa substitution for aa 394-434. Over aa 16-312, human FCRL6 shares only 33% aa identity with the mouse counterpart to FCRL6.

• Long Name:

  Fc Receptor-like 6

• Entrez Gene IDs:

  343413 (Human); 677296 (Mouse)

• Alternate Names:

  Fc receptor-like 6; FcRH6; FCRL6; FLJ16056; IFGP6