• Species Reactivity

  Human

• Specificity

  Detects human EDA‑A2/Ectodysplasin A2 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhLIGHT, rhOX40 Ligand, and rhTRAIL is observed and less than 2% cross‑reactivity with recombinant mouse EDA, rhTNF-alpha, rhVEGI, rhGITR Ligand, and rhFasLigand is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human EDA‑A2/Ectodysplasin A2    
  Ala179-Ser389    
  Accession # NP_001005609

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Human EDA‑A2/Ectodysplasin A2 (Catalog # )

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human EDA-A2 (Catalog # ) to immobilized Recombinant Human XEDAR Fc Chimera (Catalog # ) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDA-A2/Ectodysplasin A2

EDA-A2, a product of the EDA gene (also known as Tabby), is a type II transmembrane protein that is a member of the TNF superfamily. Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain.

• Entrez Gene IDs:

  1896 (Human); 13607 (Mouse)

• Alternate Names:

  EDAA2; EDA-A2