• Species Reactivity

  Human

• Specificity

  Detects human MD-1 in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse    
  MD‑1 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human MD-1    
  Gly21-Ser162    
  Accession # O95711

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.01 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Human MD‑1 (Catalog # )

• 
  

• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Daudi human Burkitt's lymphoma cell line

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Immersion fixed paraffin-embedded sections of human spleen

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MD-1

MD-1 is a secreted glycoprotein that is associated with RP105 and is required for efficient RP105 cell surface expression and function (1‑4). RP105 is a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, RP105 has a short cytoplasmic tail and lacks the Toll-IL-1 R (TIR) domain that defines the IL-1 R/TLR superfamily (1‑3). RP105 plays an important role in B-cell activation by bacterial lipopolysaccharide (LPS). It is expressed primarily on mature B cells, dendritic cells and macrophages (3).

Human MD-1 cDNA encodes a 162 amino acid (aa) precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 38% and 66% amino acid sequence identity with chicken and mouse MD-1 respectively (1, 2). MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney. The cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation and upregulate B7.2, a co-stimulatory molecule (4, 5). Since MD-1 is also expressed in liver and brain where RP105 is absent, MD-1 may also be associated with other LRR-containing molecules, or have additional functions outside the immune system (5).

• References:

1. Miyake, K. et al. (1998) J. Immun. 161:1348.

2. Miura, Y. et al. (1998) Blood 92:2815.

3. Miyake, K. et al. (1995) J. Immunol. 154:3333.

4. Nagai, Y. et al. (2002) Blood 99:1699.

5. Ogata, H. et al. (2000) J. Exp. Med. 192:23.

• Long Name:

  Myeloid Differentiation Protein 1/Lymphocyte Antigen 86

• Entrez Gene IDs:

  9450 (Human); 17084 (Mouse)

• Alternate Names:

  LY86; Ly-86; lymphocyte antigen 86; MD1; MD-1; MMD-1; Protein MD-1; RP105-associated